Eurasian Journal of Medical and Natural Sciences 4-jild 12-son (2024) · 77–84-betlar
EXTRACTION OF LECTIN FROM NATURAL RAW MATERIALS AND STUDY OF ITS PHYSICOCHEMICAL PROPERTIES
Rashidova, Nodira, Tashmuhammedova, Shohista
Annotatsiya
Lectin was extracted from Phaseolus vulgaris seeds and purified using Affi-gel blue gel, fast protein liquid chromatography (FPLC) – ion-exchange chromatography, and FPLC-gel filtration methods. The lectin consists of two 30 kDa subunits, and its N-terminal structure was found to be similar to other Phaseolus lectins. The hemagglutination activity of the lectin remained stable within a pH range of 1-14 and a temperature range of 0-80°C. The lectin strongly inhibited the proliferation of MCF-7 (breast cancer) cells with an IC50 of 1.3 μM and suppressed HIV-1 reverse transcriptase activity with an IC50 of 7.6 μM. The lectin also exhibited activity by increasing [3H-methyl]-thymidine incorporation in mouse splenocytes in response to a mitogenic stimulus. However, it did not stimulate nitric oxide production in murine peritoneal macrophages. Chemical modification results indicated that tryptophan is crucial for the hemagglutination activity of the lectin.
Lectin, Phaseolus vulgaris, protein purification, Affi-gel blue gel, FPLC (fast protein liquid chromatography), ion-exchange chromatography, gel filtration, subunits, N-terminal structure, hemagglutination activity, pH and temperature stability, MCF-7 cells, breast cancer, HIV-1 reverse transcriptase, inhibition, mitogenic response, thymidine incorporation, macrophages, nitric oxide production, chemical modification, tryptophan, and activity.Лектин, Phaseolus vulgaris, очистка белков, Affi-gel blue gel, FPLC (быстрая жидкостная хроматография белков), ионообменная хроматография, гелевая фильтрация, субъединицы, N-концевая структура, гемагглютинационная активность, стабильность pH и температуры, клетки MCF-7, рак молочной железы, обратная транскриптаза ВИЧ-1, ингибиция, митогенный ответ, включение тимидина, макрофаги, продукция оксида азота, химическая модификация, триптофан и активность.Lektin, Phaseolus vulgaris, oqsillarni tozalash, Affi-gel blue gel, FPLC (Tez oqsillar suyuqli xromatografiyasi), ion almash xromatografiyasi, gel filtratsiyasi, subbirliklar, N-terminal tuzilishi, gemagglutinatsiya faolligi, pH va harorat barqarorligi, MCF-7 hujayralari, ko‘krak bezi saratoni, HIV-1 teskari transkriptaza, ingibitsiya, mitogenik javob, timidin singishi, makrofaglar, azot oksidi hosil bo‘lishi, kimyoviy modifikatsiya, triptofan va faollik.
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